U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX5531231: WGS of Klebsiella pneumoniae:AS000879-HiSeq-2000
1 ILLUMINA (Illumina HiSeq 2000) run: 1.9M spots, 389.3M bases, 238.4Mb downloads

Design: Quality control of isolated bacterial DNA was performed using a Qubit 2.0 Fluorometer (Qubit dsDNA BR Assay Kit, Life Technologies) and an Agilent 2200 TapeStation (Genomic DNA ScreenTape, Agilent Technolgies) before library preparation. NGS libraries were prepared in 96 well format using NexteraXT DNA Sample Preparation Kit and NexteraXT index kit for 96 indexes (Illumina) according to the manufacturers protocol. The generated sequencing libraries were quantified with the qPCR-based approach using the KAPA SYBR FAST qPCR MasterMix Kit (Peqlab) on a ViiA 7 Real-Time PCR System (Life Technologies). 50 samples were pooled by lane for paired-end sequencing (2x 101 bp) on Illumina HiSeq 2000 or HiSeg 2500 using TruSeq PE cluster v3 and TruSeq SBS v3 sequencing chemistry (Illumina).
Submitted by: Ares Genetics GmbH
Study: Klebsiella pneumoniae & Escherichia coli genome sequencing and assembly
show Abstracthide Abstract
Evaluation of DNBSEQ for clinical isolate sequencing and identification of antibiotic resistance.
Sample: AS000879
SAMN11125719 • SRS4497495 • All experiments • All runs
Library:
Name: AS000879-illumina
Instrument: Illumina HiSeq 2000
Strategy: WGS
Source: GENOMIC
Selection: Restriction Digest
Layout: PAIRED
Runs: 1 run, 1.9M spots, 389.3M bases, 238.4Mb
Run# of Spots# of BasesSizePublished
SRR87384281,927,062389.3M238.4Mb2019-04-18

ID:
7461894

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...